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| Title: |  | CRYSTAL STRUCTURE OF A COMPLEX BETWEEN WT BACTERIOPHAGE MS2 COAT PROTEIN AND AN F5 APTAMER RNA STEMLOOP WITH 2AMINOPURINE SUBSTITUTED AT THE-10 POSITION |  |
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| Molecular Description: | coat protein |
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| Structural Features: | SINGLE STRAND, hairpin loop, bulge loop |
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| Nucleic Acid Sequence: |
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| Chains | R, S: | |
C C G G (2PR) G G A U C A C C A C G G
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| Protein Sequence: |
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| Primary Citation: | Horn, W.T., Convery, M.A., Stonehouse, N.J., Adams, C.J., Liljas, L., Phillips, S.E., Stockley, P.G.
The crystal structure of a high affinity RNA stem-loop complexed with the bacteriophage MS2 capsid: further challenges in the modeling of ligand-RNA interactions.
Rna
, 10,
pp. 1776 - 1782, 2004.
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| Experimental Information: | X-RAY DIFFRACTION |
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| Space Group: |
H
3
2
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| Cell Constants: |
| a = 288.610 | b = 288.610 | c = 654.680 | (Ångstroms) |
= 90.00 | = 90.00 | = 120.00 | (degrees) |
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| Crystallization Conditions: |
| Method: | | VAPOR DIFFUSION, HANGING DROP |
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| Refinement: | The structure was refined using the X-PLOR 3.851 program.
The R value is
18.8
for 170082 reflections
in the resolution range 30.0 to
2.85 Ångstroms
with Fobs > 0.0 sigma(Fobs).
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