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| Title: | | STRUCTURAL BASIS FOR 5'-END-SPECIFIC RECOGNITION OF THE GUIDE RNA STRAND BY THE A. FULGIDUS PIWI PROTEIN | |
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| Molecular Description: | hypothetical protein AF1318/RNA Complex |
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| Structural Features: | A DOUBLE HELIX, OVERHANGING BASES |
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| Nucleic Acid Sequence: |
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| Chains | C, E: | |
A G A C A G
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| Chains | D, F: | |
U G U C
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| Protein Sequence: |
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| Primary Citation: | Ma, J.B., Yuan, Y.R., Meister, G., Pei, Y., Tuschl, T., Patel, D.J.
Structural basis for 5'-end-specific recognition of guide RNA by the A. fulgidus Piwi protein.
Nature
, 434,
pp. 666 - 670, 2005.
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| Experimental Information: | X-RAY DIFFRACTION |
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| Space Group: |
H
3
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| Cell Constants: |
| a = 238.747 | b = 238.747 | c = 52.042 | (Ångstroms) |
 = 90.00 |  = 90.00 |  = 120.00 | (degrees) |
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| Crystallization Conditions: |
| Method: | | VAPOR DIFFUSION, HANGING DROP |
| Drop: | | PEG
4K potassium chloride, magnisium chloride, cacodylate, H2O |
| Reservoir: | | potassium chloride, magnisium chloride, PEG
4K |
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| Refinement: | The structure was refined using the REFMAC 5.2.0005 program.
The R value is
20.392
for 36055 reflections
in the resolution range 50 to
2.50 Ångstroms
with Fobs > 2 sigma(Fobs).
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