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| Title: | | RO AUTOANTIGEN COMPLEXED WITH RNAS | |
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| Molecular Description: |
60-kDa SS-A/Ro ribonucleoprotein/RNA complex |
 |
| Structural Features: | A DOUBLE HELIX, OVERHANGING BASES |
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| Nucleic Acid Sequence: |
| | | |
| Chains | C, E, G, H: | |
G C (IU) G G U C C G A
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| Chains | D, F: | |
(IU) (IU) G A C (IU) A G C C
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| Protein Sequence: |
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| Primary Citation: | Stein, A.J., Fuchs, G., Fu, C., Wolin, S.L., Reinisch, K.M.
Structural Insights into RNA Quality Control: The Ro Autoantigen Binds Misfolded RNAs via Its Central Cavity
Cell (Cambridge,Mass.)
, 121,
pp. 529 - 539, 2005.
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| | | |
| Experimental Information: | X-RAY DIFFRACTION |
|
| Space Group: |
P
1
21
1
|
|
| Cell Constants: |
| a = 60.30 | b = 153.65 | c = 80.56 | (Ångstroms) |
 = 90 |  = 92.8 |  = 90 | (degrees) |
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| Crystallization Conditions: |
| Method: | | VAPOR DIFFUSION, HANGING DROP |
| Drop: | | MPD, sodium acetate, magnesium acetate, DTT |
| Reservoir: | | MPD, sodium acetate, magnesium acetate, DTT |
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| Refinement: | The structure was refined using the CNS 1.1 program.
The R value is
20.2
for 70300 reflections
in the resolution range 20. to
2.20 Ångstroms
with Fobs > 0.0 sigma(Fobs).
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