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| Title: | | COUPLING OF ACTIVE SITE MOTIONS AND RNA BINDING | |
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| Molecular Description: |
CRYSTAL STRUCTURE OF THE E. COLI PSEUDOURIDINE SYNTHASE TRUB MUTANT D48N BOUND TO A T STEM-LOOP RNA |
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| Structural Features: | T DOUBLE HELIX, HAIRPIN LOOP |
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| Nucleic Acid Sequence: |
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| Chain | B: | | G G C A A C G G U (UD5) C G A U C C C G U U G C |
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| Protein Sequence: |
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| Primary Citation: | Hoang, C., Hamilton, C.S., Mueller, E.G., Ferre-D'Amare, A.R.
Precursor complex structure of pseudouridine synthase TruB suggests coupling of active site perturbations to an RNA-sequestering peripheral protein domain
Protein Sci.
, 14,
pp. 2201 - 2206, 2005.
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| Experimental Information: | X-RAY DIFFRACTION |
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| Space Group: |
C
1
2
1
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| Cell Constants: |
| a = 145.433 | b = 40.753 | c = 78.803 | (Ångstroms) |
 = 90.00 |  = 110.61 |  = 90.00 | (degrees) |
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| Refinement: | The structure was refined using the CNS 1.0 program.
The R value is
20.2
for 10935 reflections
in the resolution range 19.92 to
2.80 Ångstroms
with Fobs > 0.3 sigma(Fobs).
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