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| Title: | | RNA STEMLOOP OPERATOR FROM BACTERIOPHAGE QBETA COMPLEXED WITH AN N87S,E89K MUTANT MS2 CAPSID | |
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| Molecular Description: | Coat protein/RNA Complex |
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| Structural Features: | SINGLE STRAND, HAIRPIN LOOP |
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| Nucleic Acid Sequence: |
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| Chains | R, S: | |
A U G C A U G U C U A A G A C A G C A U
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| Protein Sequence: |
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| Primary Citation: | Horn, W.T., Tars, K., Grahn, E., Helgstrand, C., Baron, A.J., Lago, H., Adams, C.J., Peabody, D.S., Phillips, S.E.V., Stonehouse, N.J., Liljas, L., Stockley, P.G.
Structural basis of RNA binding discrimination between bacteriophages Qbeta and MS2
Structure
, 14,
pp. 487 - 495, 2006.
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| Experimental Information: | X-RAY DIFFRACTION |
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| Space Group: |
H
3
2
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| Cell Constants: |
| a = 287.80 | b = 287.80 | c = 652.60 | (Ångstroms) |
 = 90 |  = 90 |  = 120 | (degrees) |
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| Crystallization Conditions: |
| Method: | | VAPOR DIFFUSION, HANGING DROP |
| Drop: | | PEG
8000 0.4M SODIUM PHOSPHATE BUFFER, water |
| Reservoir: | | PEG
8000 0.4M SODIUM PHOSPHATE BUFFER, water |
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| Refinement: | The structure was refined using the CNS 1.1 program.
The R value is
19.7
for 147903 reflections
in the resolution range 30 to
2.9 Ångstroms
with I > 0.0 sigma(I).
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