 | | | |
| Title: | | RNA STEMLOOP FROM BACTERIOPHAGE MS2 COMPLEXED WITH AN N87S,E89K MUTANT MS2 CAPSID | |
|
| Molecular Description: | Coat protein/RNA |
 |
| Structural Features: | SINGLE STRAND, HAIRPIN LOOP |
|
| Nucleic Acid Sequence: |
| | | |
| Chains | R, S: | |
A C A U G A G G A U U A C C C A U G U
|
|
|
| Protein Sequence: |
|
|
| Primary Citation: | Horn, W.T., Tars, K., Grahn, E., Helgstrand, C., Baron, A.J., Lago, H., Adams, C.J., Peabody, D.S., Phillips, S.E.V., Stonehouse, N.J., Liljas, L., Stockley, P.G.
Structural basis of RNA binding discrimination between bacteriophages Qbeta and MS2
Structure
, 14,
pp. 487 - 495, 2006.
|
|
| | | |
| Experimental Information: | X-RAY DIFFRACTION |
|
| Space Group: |
H
3
2
|
|
| Cell Constants: |
| a = 288.062 | b = 288.062 | c = 652.812 | (Ångstroms) |
 = 90.00 |  = 90.00 |  = 120.00 | (degrees) |
|
|
| Crystallization Conditions: |
| Method: | | VAPOR DIFFUSION, HANGING DROP |
| Drop: | | PEG
8000 0.4M SODIUM PHOSPHATE BUFFER |
| Reservoir: | | PEG
8000 0.4M SODIUM PHOSPHATE BUFFER |
|
|
| Refinement: | The structure was refined using the CNS 1.1 program.
The R value is
22.7
for 123697 reflections
in the resolution range 30.0 to
3.15 Ångstroms
with I > 0.0 sigma(I).
|
|
|
