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| Title: |  | GLUTAMINYL-TRNA SYNTHETASE MUTANT I129T COMPLEXED WITH GLUTAMINE TRANSFER RNA |  |
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| Molecular Description: |
GLUTAMINYL-TRNA SYNTHETASE (E.C.6.1.1.18) MUTANT/RNA COMPLEX + ADENOSINE-5'- TRIPHOSPHATE |
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| Structural Features: | T SINGLE STRAND, LOOP |
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| Nucleic Acid Sequence: |
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| Chain | B: | | U G G G G U A U C G C C A A G C G G U A A G G C A C C G G A U U C U G A U U C C G G C A U U C C G A G G U U C G A A U C C U C G U A C C C C A G C C A |
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| Protein Sequence: |
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| Primary Citation: | Arnez, J.G., Steitz, T.A.
Crystal structures of three misacylating mutants of Escherichia coli glutaminyl-tRNA synthetase complexed with tRNA(Gln) and ATP.
Biochemistry
, 35,
pp. 14725 - 14733, 1996.
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| Experimental Information: | X-RAY DIFFRACTION |
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| Space Group: |
C
2
2
21
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| Cell Constants: |
| a = 242.000 | b = 93.700 | c = 115.500 | (Ångstroms) |
= 90.00 | = 90.00 | = 90.00 | (degrees) |
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| Refinement: | The structure was refined using the X-PLOR 3.1 program.
The R value is
23.0
for 22183 reflections
in the resolution range 7.000 to
3.000 Ångstroms
with Fobs > 0.000 sigma(Fobs).
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