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| Title: | | CRYSTAL STRUCTURE ANALYSIS OF A TRNA-NEOMYCIN COMPLEX | |
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| Molecular Description: | PHENYLALANINE TRANSFER RNA
NEOMYCIN |
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| Structural Features: | T SINGLE STRAND |
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| Nucleic Acid Sequence: |
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| Chain | A: | |
G C G G A U U U A G C U C A G U U G G G A G A G C G C C A G A C U G A A (YG) A U C U G G A G G U C C U G U G (5MU) U C G A U C C A C A G A A U U C G C A
C C A
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| Primary Citation: | Mikkelsen, N.E., Johansson, K., Virtanen, A., Kirsebom, L.A.
Aminoglycoside binding displaces a divalent metal ion in a tRNA-neomycin B complex.
Nat.Struct.Biol.
, 8,
pp. 510 - 514, 2001.
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| Experimental Information: | X-RAY DIFFRACTION |
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| Space Group: |
P
1
21
1
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| Cell Constants: |
| a = 54.900 | b = 32.900 | c = 63.100 | (Ångstroms) |
 = 90.00 |  = 90.50 |  = 90.00 | (degrees) |
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| Crystallization Conditions: |
| Method: | | VAPOR DIFFUSION, SITTING DROP |
| Drop: | | MgCl, MPD, Cacodylate |
| Reservoir: | | MPD |
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| Refinement: | The structure was refined using the CNS 1.0 program.
The R value is
22.8
for 6515 reflections
in the resolution range 20 to
2.6 Ångstroms
with I > 0 sigma(I).
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