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| Title: |  | HOLLIDAY JUNCTION TCGGTACCGA WITH NA AND CA BINDING SITES. |  |
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| Molecular Description: | 5'-D(TpCpGpGpTpApCpCpGpA)-3' |
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| Structural Features: | DOUBLE HELIX, HOLLIDAY JUNCTION |
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| Nucleic Acid Sequence: |
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| Chains | A, B, C, D: | |
(DT) (DC) (DG) (DG) (DT) (DA) (DC) (DC) (DG) (DA)
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| Primary Citation: | Thorpe, J.H., Gale, B.C., Teixeira, S.C.M., Cardin, C.J.
Conformational and Hydration Effects of Site-selective Sodium, Calcium and Strontium Ion Binding to the DNA Holliday Junction Structure d(TCGGTACCGA)4
J.Mol.Biol.
, 327,
pp. 97 - 109, 2003.
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| Experimental Information: | X-RAY DIFFRACTION |
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| Space Group: |
C
1
2
1
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| Cell Constants: |
| a = 66.067 | b = 23.775 | c = 73.816 | (Ångstroms) |
= 90.00 | = 110.58 | = 90.00 | (degrees) |
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| Crystallization Conditions: |
| Method: | | VAPOR DIFFUSION, SITTING DROP |
| Drop: | | Sodium Cacodylate, Calcium Chloride, MPD |
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| Refinement: | The structure was refined using the REFMAC 5 program.
The R value is
22.821
for 8780 reflections
in the resolution range 15.00 to
1.85 Ångstroms
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