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| Title: | | EFFECT OF SEQUENCE ON THE CONFORMATIONAL GEOMETRY OF DNA HOLLIDAY JUNCTIONS | |
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| Molecular Description: | 5'-D(CpCpGpGpCpGpCpCpGpG)-3' |
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| Structural Features: | B DOUBLE HELIX, Flipped-out bases |
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| Nucleic Acid Sequence: |
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| Chains | A, B: | |
(DC) (DC) (DG) (DG) (DC) (DG) (DC) (DC) (DG) (DG)
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| Primary Citation: | Hays, F.A., Vargason, J.M., Ho, P.S.
Effect of Sequence on the Conformation of DNA Holliday Junctions
Biochemistry
, 42,
pp. 9586 - 9597, 2003.
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| Experimental Information: | X-RAY DIFFRACTION |
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| Space Group: |
C
1
2
1
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| Cell Constants: |
| a = 66.513 | b = 24.179 | c = 37.004 | (Ångstroms) |
 = 90.0 |  = 110.010 |  = 90.0 | (degrees) |
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| Crystallization Conditions: |
| Drop: | | sodium cacodylate, CaCl2, SPERMINE TETRAHYDROCHLORIDE, H2O |
| Reservoir: | | sodium cacodylate, CaCl2, SPERMINE TETRAHYDROCHLORIDE, H2O |
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| Refinement: | The structure was refined using the CNS 1.0 program.
The R value is
22.9
for 4436 reflections
in the resolution range 22.50 to
1.70 Ångstroms
with Fobs > 2.000 sigma(Fobs).
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