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| Title: | | EFFECT OF SEQUENCE ON THE CONFORMATIONAL GEOMETRY OF DNA HOLLIDAY JUNCTIONS | |
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| Molecular Description: | 5'-D(CpCpApGpTpApCp(BRU)pGpG)-3' |
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| Structural Features: | B DOUBLE HELIX, OVERHANGING BASES |
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| Nucleic Acid Sequence: |
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| Chains | A, B: | |
(DC) (DC) (DA) (DG) (DT) (DA) (DC) (BRU) (DG) (DG)
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| Primary Citation: | Hays, F.A., Vargason, J.M., Ho, P.S.
Effect of Sequence on the Conformation of DNA Holliday Junctions
Biochemistry
, 42,
pp. 9586 - 9597, 2003.
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| Experimental Information: | X-RAY DIFFRACTION |
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| Space Group: |
C
1
2
1
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| Cell Constants: |
| a = 64.840 | b = 22.580 | c = 38.070 | (Ångstroms) |
 = 90.00 |  = 106.78 |  = 90.00 | (degrees) |
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| Crystallization Conditions: |
| Drop: | | Tris-HCL, calcium acetate, 2-methyl-2,4-pentane diol, H2O |
| Reservoir: | | calcium acetate, Tris-HCL, 2-methyl-2,4-pentane diol, H2O |
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| Refinement: | The structure was refined using the CNS 1.0 program.
The R value is
21.5
for 3745 reflections
in the resolution range 19.18 to
1.90 Ångstroms
with Fobs > 0.000 sigma(Fobs).
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