 | | | |
| Title: | | CRYSTAL STRUCTURE OF D(GCGATAGC): THE BASE-INTERCALATED DUPLEX | |
|
| Molecular Description: | 5'-D(Gp(CBR)pGpApTpApGpC)-3' |
 |
| Structural Features: | RH DOUBLE HELIX, BASE INTERCALATED |
|
| Nucleic Acid Sequence: |
| | | |
| Chain | A: | |
(DG) (CBR) (DG) (DA) (DT) (DA) (DG) (DC)
|
|
|
| Primary Citation: | Kondo, J., Umeda, S.I., Fujita, K., Sunami, T., Takenaka, A.
X-ray analyses of d(GCGAXAGC) containing G and T at X: the base-intercalated duplex is still stable even in point mutants at the fifth residue.
J.Synchrotron Radiat.
, 11,
pp. 117 - 120, 2004.
|
|
| | | |
| Experimental Information: | X-RAY DIFFRACTION |
|
| Space Group: |
P
63
2
2
|
|
| Cell Constants: |
| a = 37.000 | b = 37.000 | c = 65.300 | (Ångstroms) |
 = 90.00 |  = 90.00 |  = 120.00 | (degrees) |
|
|
| Crystallization Conditions: |
| Method: | | VAPOR DIFFUSION, HANGING DROP |
| Drop: | | 2-methyl-2,4-pentandiol, hexamine cobalt chloride, magnesium chloride, sodium cacodylate |
| Reservoir: | | hexamine cobalt chloride, magnesium chloride, sodium cacodylate |
|
|
| Refinement: | The structure was refined using the CNS 1.0 program.
The R value is
22.4
for 2707 reflections
in the resolution range 22.87 to
1.7 Ångstroms
with Fobs > 3.0 sigma(Fobs).
|
|
|
