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| Title: | | THE THREE-DIMENSIONAL STRUCTURES OF BULGE-CONTAINING DNA FRAGMENTS | |
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| Molecular Description: | 5'-D(CpGpCpApGpApApTpTpCpGpCpG)-3' |
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| Structural Features: | B DOUBLE HELIX, BULGE, FLIPPED-OUT BASE |
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| Nucleic Acid Sequence: |
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| Chains | A, B: | |
(DC) (DG) (DC) (DA) (DG) (DA) (DA) (DT) (DT) (DC) (DG) (DC) (DG)
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| Primary Citation: | Joshua-Tor, L., Frolow, F., Appella, E., Hope, H., Rabinovich, D., Sussman, J.L.
Three-dimensional structures of bulge-containing DNA fragments.
J.Mol.Biol.
, 225,
pp. 397 - 431, 1992.
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| Experimental Information: | X-RAY DIFFRACTION |
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| Space Group: |
C
1
2
1
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| Cell Constants: |
| a = 78.480 | b = 42.840 | c = 25.160 | (Ångstroms) |
 = 90.00 |  = 99.36 |  = 90.00 | (degrees) |
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| Crystallization Conditions: |
| Method: | | VAPOR DIFFUSION, SITTING DROP |
| Drop: | | WATER, MPD, SPERMINE, MgCl2, Na Cacodylate |
| Reservoir: | | WATER, MPD, NaCl |
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| Refinement: | The structure was refined using the NUCLSQ, X-PLOR program.
The R value is
18.6
for 1640 reflections
in the resolution range 10.000 to
2.600 Ångstroms
with Fobs > 3.000 sigma(Fobs).
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