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| Title: |  | CRYSTAL STRUCTURES OF AN A-FORM DUPLEX WITH SINGLE-ADENOSINE BULGES AND A CONFORMATIONAL BASIS FOR SITE SPECIFIC RNA SELF-CLEAVAGE |  |
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| Molecular Description: | 5'-R(GpCpGp)-D(ApTpApTpAp)-R(CpGpC)-3' ORTHORHOMBIC |
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| Structural Features: | A DOUBLE HELIX, BULGE |
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| Nucleic Acid Sequence: |
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| Primary Citation: | Portmann, S., Grimm, S., Workman, C., Usman, N., Egli, M.
Crystal structures of an A-form duplex with single-adenosine bulges and a conformational basis for site-specific RNA self-cleavage.
Chem.Biol.
, 3,
pp. 173 - 184, 1996.
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| Experimental Information: | X-RAY DIFFRACTION |
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| Space Group: |
C
2
2
21
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| Cell Constants: |
| a = 25.890 | b = 33.970 | c = 57.560 | (Ångstroms) |
= 90.00 | = 90.00 | = 90.00 | (degrees) |
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| Crystallization Conditions: |
| Method: | | VAPOR DIFFUSION |
| Drop: | | WATER, Na Cacodylate, SPERMINE, MgCl2 |
| Reservoir: | | WATER, MPD |
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| Refinement: | The structure was refined using the X-PLOR program.
The R value is
15.9
for 2170 reflections
in the resolution range 10.000 to
1.830 Ångstroms
with Fobs > 2.000 sigma(Fobs).
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