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| Title: | | THE CRYSTAL STRUCTURE OF A D-LYSINE-BASED CHIRAL PNA-DNA DUPLEX | |
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| Molecular Description: | DNA/PEPTIDE NUCLEIC ACID COMPLEX |
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| Structural Features: | RH DOUBLE HELIX |
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| Nucleic Acid Sequence: |
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| Chain | A: | |
(DA) (DG) (DT) (DG) (DA) (DT) (DC) (DT) (DA) (DC)
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| Chain | B: | |
(GPN) (TPN) (APN) (GPN) (A66) (T66) (C66) (APN) (CPN) (TPN)
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| Primary Citation: | Menchise, V., De Simone, G., Tedeschi, T., Corradini, R., Sforza, S., Marchelli, R., Capasso, D., Saviano, M., Pedone, C.
Insights into peptide nucleic acid (PNA) structural features: The crystal structure of a D-lysine-based chiral PNA-DNA duplex
Proc.Natl.Acad.Sci.USA
, 100,
pp. 12021 - 12026, 2003.
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| Experimental Information: | X-RAY DIFFRACTION |
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| Space Group: |
P
31
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| Cell Constants: |
| a = 34.94 | b = 34.94 | c = 35.80 | (Ångstroms) |
 = 90.00 |  = 90.00 |  = 120.00 | (degrees) |
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| Crystallization Conditions: |
| Method: | | VAPOR DIFFUSION, HANGING DROP |
| Drop: | | AMMONIUM ACETATE, PEG
8000 Na CACODYLATE, MgCl2, MES, ISOPROPANOL |
| Reservoir: | | AMMONIUM ACETATE, PEG
8000 MgCl2, ISOPROPANOL |
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| Refinement: | The structure was refined using the CNS 1.1 program.
The R value is
19.7
for 5429 reflections
in the resolution range 20.0 to
1.66 Ångstroms
with Fobs > 0 sigma(Fobs).
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