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| Title: | | CRYSTAL STRUCTURE OF A LEADZYME; METAL BINDING AND IMPLICATIONS FOR CATALYSIS | |
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| Molecular Description: | 5'-R(CpGpGpApCpCpGpApGpCpCpApG)-3'
5'-R(GpCpUpGpGpGpApGp UpCpC)-3' |
 |
| Structural Features: | DOUBLE HELIX, FLIPPED-OUT BASES, OVERHANGING BASES |
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| Nucleic Acid Sequence: |
| | | |
| Chains | A, C: | |
C G G A C C G A G C C A G
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| Chains | B, D: | |
G C U G G G A G U C C
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| Primary Citation: | Wedekind, J.E., McKay, D.B.
Crystal structure of a lead-dependent ribozyme revealing metal binding sites relevant to catalysis.
Nat.Struct.Biol.
, 6,
pp. 261 - 268, 1999.
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| | | |
| Experimental Information: | X-RAY DIFFRACTION |
|
| Space Group: |
P
61
2
2
|
|
| Cell Constants: |
| a = 60.400 | b = 60.400 | c = 133.100 | (Ångstroms) |
 = 90.00 |  = 90.00 |  = 120.00 | (degrees) |
|
|
| Crystallization Conditions: |
| Method: | | VAPOR DIFFUSION, HANGING DROP |
| Drop: | | MAGNESIUM ACETATE, SODIUM CACODYLATE, SPERMINE, MPD |
| Reservoir: | | MPD |
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| Refinement: | The structure was refined using the CNS program.
The R value is
25.5
for 4169 reflections
in the resolution range 25.0 to
2.70 Ångstroms
. |
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