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| Title: | | CRYSTAL STRUCTURE ANALYSIS OF A MUTANT P4-P6 DOMAIN (DELC209) OF TETRAHYMENA THEMOPHILA GROUP I INTRON. | |
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| Molecular Description: | P4-P6 MUTANT RNA RIBOZYME DOMAIN |
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| Structural Features: | SINGLE STRAND, LOOP |
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| Nucleic Acid Sequence: |
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| Primary Citation: | Juneau, K., Podell, E., Harrington, D.J., Cech, T.R.
Structural basis of the enhanced stability of a mutant ribozyme domain and a detailed view of RNA--solvent interactions.
Structure
, 9,
pp. 221 - 231, 2001.
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| Experimental Information: | X-RAY DIFFRACTION |
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| Space Group: |
P
21
21
21
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| Cell Constants: |
| a = 75.300 | b = 125.400 | c = 145.700 | (Ångstroms) |
 = 90.00 |  = 90.00 |  = 90.00 | (degrees) |
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| Crystallization Conditions: |
| Method: | | VAPOR DIFFUSION, SITTING DROP |
| Drop: | | MPD, MgCl2, sodium cacodylate, spermine, NaCl |
| Reservoir: | | MPD |
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| Refinement: | The structure was refined using the CNS program.
The R value is
24.4
for 60667 reflections
in the resolution range 30 to
2.25 Ångstroms
with I > 0 sigma(I).
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