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| Title: | | CRYSTAL STRUCTURE OF A RNA MOLECULE CONTAINING DOMAIN 5 AND 6 OF THE YEAST AI5G GROUP II SELF-SPLICING INTRON | |
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| Molecular Description: | ai5g group II Self-splicing intron |
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| Structural Features: | SINGLE STRAND, LOOP, BULGE |
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| Nucleic Acid Sequence: |
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| Chain | A: | |
G U C U A C C U A U C G G G C U A A G G A G C C G U A U G C G A U G A A A G U C G C A C G U A C G G U U C U A U G C C C G G G G G A A A A C
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| Primary Citation: | Zhang, L., Doudna, J.A.
Structural insights into group II intron catalysis and branch-site selection.
Science
, 295,
pp. 2084 - 2088, 2002.
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| Experimental Information: | X-RAY DIFFRACTION |
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| Space Group: |
P
61
2
2
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| Cell Constants: |
| a = 90.987 | b = 90.987 | c = 235.891 | (Ångstroms) |
 = 90.00 |  = 90.00 |  = 120.00 | (degrees) |
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| Crystallization Conditions: |
| Method: | | VAPOR DIFFUSION, SITTING DROP |
| Drop: | | HEPES-KOH, MgCl, ammonium sulfate, spermine |
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| Refinement: | The structure was refined using the CNS program.
The R value is
24.76
for 10014 reflections
in the resolution range 30 to
3.0 Ångstroms
with Fobs > 0.0 sigma(Fobs).
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