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| Title: | | CROSSLINKED HAMMERHEAD RIBOZYME INITIAL STATE | |
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| Molecular Description: | Hammerhead Ribozyme |
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| Structural Features: | DOUBLE HELIX, bulge, flipped-out bases, overhanging bases |
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| Nucleic Acid Sequence: |
| | | |
| Chain | A: | |
G U G G U C U G A U G A G G C C
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| Chain | B: | |
G C C G A A A C U C G U A A G A G U C A C C A C
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| Primary Citation: | Dunham, C.M., Murray, J.B., Scott, W.G.
A helical twist-induced conformational switch activates cleavage in the hammerhead ribozyme.
J.Mol.Biol.
, 332,
pp. 327 - 336, 2003.
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| | | |
| Experimental Information: | X-RAY DIFFRACTION |
|
| Space Group: |
P
31
2
1
|
|
| Cell Constants: |
| a = 65.639 | b = 65.639 | c = 137.475 | (Ångstroms) |
 = 90.00 |  = 90.00 |  = 120.00 | (degrees) |
|
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| Crystallization Conditions: |
| Method: | | VAPOR DIFFUSION, HANGING DROP |
| Drop: | | LiSO4, sodium cacodylate |
| Reservoir: | | LiSO4 |
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| Refinement: | The structure was refined using the REFMAC 5.1 program.
The R value is
20.332
for 7544 reflections
in the resolution range 19.76 to
2.85 Ångstroms
with Fobs > 0.0 sigma(Fobs).
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