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| Title: | | STRUCTURE OF THE TETRAHYMENA RIBOZYME: BASE TRIPLE SANDWICH AND METAL ION AT THE ACTIVE SITE | |
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| Molecular Description: | Tetrahymena ribozyme RNA |
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| Structural Features: | SINGLE STRAND, HAIRPIN LOOP, LOOPS |
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| Nucleic Acid Sequence: |
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| Primary Citation: | Guo, F., Gooding, A.R., Cech, T.R.
Structure of the Tetrahymena ribozyme: base triple sandwich and metal ion at the active site.
Mol.Cell
, 16,
pp. 351 - 362, 2004.
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| Experimental Information: | X-RAY DIFFRACTION |
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| Space Group: |
P
41
21
2
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| Cell Constants: |
| a = 175.400 | b = 175.400 | c = 304.800 | (Ångstroms) |
 = 90.00 |  = 90.00 |  = 90.00 | (degrees) |
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| Crystallization Conditions: |
| Method: | | VAPOR DIFFUSION, HANGING DROP |
| Drop: | | MPD, cacodylate, KCl, NaCl, MgCl2, spermine, H2O |
| Reservoir: | | cacodylate, KCl, NaCl, MgCl2, H2O |
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| Refinement: | The structure was refined using the CNS program.
The R value is
26.3
for 43972 reflections
in the resolution range 20 to
3.8 Ångstroms
with Fobs > 0 sigma(Fobs).
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