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| Title: | | CRYSTAL STRUCTURE OF DIELS-ALDER RIBOZYME | |
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| Molecular Description: | ribozyme |
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| Structural Features: | SINGLE STRAND, LOOP, HAIRPIN LOOP, HAMMERHEAD DNA-RNA RIBOZYME |
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| Nucleic Acid Sequence: |
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| Chains | A, C: | |
G G A G C U C G C C C
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| Chains | B, D: | |
G G G C G A G G C C G U G C C A G C U C U U C G G A G C A A U A C U C G G C
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| Primary Citation: | Serganov, A., Keiper, S., Malinina, L., Tereshko, V., Skripkin, E., Hobartner, C., Polonskaia, A., Phan, A.T., Wombacher, R., Micura, R., Dauter, Z., Jaschke, A., Patel, D.J.
Structural basis for Diels-Alder ribozyme-catalyzed carbon-carbon bond formation.
Nat.Struct.Mol.Biol.
, 12,
pp. 218 - 224, 2005.
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| Experimental Information: | X-RAY DIFFRACTION |
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| Space Group: |
P
1
21
1
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| Cell Constants: |
| a = 77.690 | b = 44.240 | c = 79.640 | (Ångstroms) |
 = 90.00 |  = 107.12 |  = 90.00 | (degrees) |
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| Crystallization Conditions: |
| Method: | | VAPOR DIFFUSION, HANGING DROP |
| Drop: | | PEG
00 sodium cacodylate, H2O |
| Reservoir: | | PEG
00 sodium cacodylate, magnesium acetate, 1,6-hexandiol, sodium chloride, cadmium chloride, H2O |
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| Refinement: | The structure was refined using the REFMAC 5.1.9999 program.
The R value is
28.57
for 6563 reflections
in the resolution range 20 to
3.5 Ångstroms
with Fobs > 0.0 sigma(Fobs).
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