 | | | |
| Title: | | CRYSTAL STRUCTURE OF THE DIELS-ALDER RIBOZYME COMPLEXED WITH THE PRODUCT OF THE REACTION BETWEEN N-PENTYLMALEIMIDE AND COVALENTLY ATTACHED 9-HYDROXYMETHYLANTHRACENE | |
|
| Molecular Description: | ribozyme
(3AS,9AS)-2-PENTYL-4-HYDROXYMETHYL-3A,4,9,9A-TETRAHYDRO-4,9[1',2']-BENZENO-1H-BENZ[F]ISOINDOLE-1,3(2H)-DIONE |
 |
| Structural Features: | SINGLE STRAND, LOOP, HAIRPIN LOOP, HAMMERHEAD DNA-RNA RIBOZYME |
|
| Nucleic Acid Sequence: |
| | | |
| Chains | A, C: | |
G G A G C U C G C C C
|
| Chains | B, D: | |
G G G C G A G G C C G U G C C A G C U C U U C G G A G C A A U A C U C G G C
|
|
|
| Primary Citation: | Serganov, A., Keiper, S., Malinina, L., Tereshko, V., Skripkin, E., Hobartner, C., Polonskaia, A., Phan, A.T., Wombacher, R., Micura, R., Dauter, Z., Jaschke, A., Patel, D.J.
Structural basis for Diels-Alder ribozyme-catalyzed carbon-carbon bond formation.
Nat.Struct.Mol.Biol.
, 12,
pp. 218 - 224, 2005.
|
|
| | | |
| Experimental Information: | X-RAY DIFFRACTION |
|
| Space Group: |
P
1
21
1
|
|
| Cell Constants: |
| a = 78.288 | b = 44.039 | c = 79.998 | (Ångstroms) |
 = 90.00 |  = 106.87 |  = 90.00 | (degrees) |
|
|
| Crystallization Conditions: |
| Method: | | VAPOR DIFFUSION, HANGING DROP |
| Drop: | | PEG
00 sodium HEPES, H2O |
| Reservoir: | | PEG
00 sodium HEPES, magnesium chloride, ethylacetate, H2O |
|
|
| Refinement: | The structure was refined using the REFMAC 5.1.9999 program.
The R value is
22.802
for 7522 reflections
in the resolution range 20.00 to
3.30 Ångstroms
with I > 0 sigma(I).
|
|
|
