 | | | |
| Title: | | THE STRUCTURE OF AN ALL-RNA MINIMAL HAIRPIN RIBOZYME WITH MUTATION G8A AT THE CLEAVAGE SITE | |
|
| Molecular Description: | RNA |
 |
| Structural Features: | DOUBLE HELIX, BULGES, FLIPPED-OUT BASES, OVERHANGING BASES, RNA HAMMERHEAD RIBOZYME |
|
| Nucleic Acid Sequence: |
| | | |
| Chain | A: | |
U C C C A G U C C A C C G
|
| Chain | B: | |
C G G U G A A A A G G G
|
| Chain | C: | |
G G C A G A G A A A C A C A C G A
|
| Chain | D: | |
U C G U G G U A C A U U A C C U G C C
|
|
|
| Primary Citation: | Salter, J., Krucinska, J., Alam, S., Grum-Tokars, V., Wedekind, J.E.
Water in the Active Site of an All-RNA Hairpin Ribozyme and Effects of Gua8 Base Variants on the Geometry of Phosphoryl Transfer.
Biochemistry
, 45,
pp. 686 - 700, 2006.
|
|
| | | |
| Experimental Information: | X-RAY DIFFRACTION |
|
| Space Group: |
P
61
2
2
|
|
| Cell Constants: |
| a = 93.510 | b = 93.510 | c = 124.550 | (Ångstroms) |
 = 90.00 |  = 90.00 |  = 120.00 | (degrees) |
|
|
| Crystallization Conditions: |
| Method: | | VAPOR DIFFUSION, HANGING DROP |
| Drop: | | PEG
2000MME LITHIUM SULFATE, SPERMIDINE, COBALT HEXAAMINE, H2O |
| Reservoir: | | LITHIUM SULFATE, H2O |
|
|
| Refinement: | The structure was refined using the CNS 1.1 program.
The R value is
24.3
for 13109 reflections
in the resolution range 43.77 to
2.40 Ångstroms
with I > -5 sigma(I).
|
|
|
