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| Title: | | THE STRUCTURE OF A MINIMAL ALL-RNA HAIRPIN RIBOZYME WITH THE MUTANT G8U AT THE CLEAVAGE SITE | |
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| Molecular Description: | RNA |
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| Structural Features: | DOUBLE HELIX, BULGES, FLIPPED-OUT BASES, OVERHANGING BASES, RNA HAMMERHEAD RIBOZYME |
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| Nucleic Acid Sequence: |
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| Chain | A: | | U C C C A G U C C A C C G |
| Chain | B: | | C G G U G A U A A G G G |
| Chain | C: | | G G C A G A G A A A C A C A C G A |
| Chain | D: | | U C G U G G U A C A U U A C C U G C C |
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| Primary Citation: | Salter, J., Krucinska, J., Alam, S., Grum-Tokars, V., Wedekind, J.E.
Water in the Active Site of an All-RNA Hairpin Ribozyme and Effects of Gua8 Base Variants on the Geometry of Phosphoryl Transfer.
Biochemistry
, 45,
pp. 686 - 700, 2006.
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| Experimental Information: | X-RAY DIFFRACTION |
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| Space Group: |
P
61
2
2
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| Cell Constants: |
| a = 93.350 | b = 93.350 | c = 123.560 | (Ångstroms) |
 = 90.00 |  = 90.00 |  = 120.00 | (degrees) |
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| Refinement: | The structure was refined using the CNS 1.1 program.
The R value is
23.7
for 13267 reflections
in the resolution range 46.68 to
2.38 Ångstroms
with I > -5. sigma(I).
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