 | | | |
| Title: | | CRYSTAL STRUCTURE OF A MINIMAL, MUTANT ALL-RNA HAIRPIN RIBOZYME (U39C, G8MTU) | |
|
| Molecular Description: | ribozyme |
 |
| Structural Features: | DOUBLE HELIX, bulges, overhanging bases, RNA HAMMERHEAD RIBOZYME |
|
| Nucleic Acid Sequence: |
| | | |
| Chain | A: | | U C C C A G U C C A C C G |
| Chain | B: | | C G G U G A (MTU) A A G G G |
| Chain | C: | | G G C A G A G A A A C A C A C G A |
| Chain | D: | | U C G U G G U A C A U U A C C U G C C |
|
|
| Primary Citation: | Salter, J.D., Krucinska, J., Alam, S., Grum-Tokars, V., Wedekind, J.E.
Water in the Active Site of an All-RNA Hairpin Ribozyme and Effects of Gua8 Base Variants on the Geometry of Phosphoryl Transfer.
Biochemistry
, 45,
pp. 686 - 700, 2006.
|
|
| | | |
| Experimental Information: | X-RAY DIFFRACTION |
|
| Space Group: |
P
61
2
2
|
|
| Cell Constants: |
| a = 93.900 | b = 93.900 | c = 129.180 | (Ångstroms) |
 = 90.00 |  = 90.00 |  = 120.00 | (degrees) |
|
|
| Refinement: | The structure was refined using the CNS 1.1 program.
The R value is
24.4
for 9550 reflections
in the resolution range 38.06 to
2.70 Ångstroms
with I > -3. sigma(I).
|
|
|
