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| Title: | | SELENIUM-ASSISTED NUCLEIC ACID CRYSTALLOGRAPHY: USE OF PHOSPHOROSELENOATES FOR MAD PHASING OF A DNA STRUCTURE | |
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| Molecular Description: | 5'-D(Cp(GMS)pCpGpCpG)-3' |
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| Structural Features: | Z DOUBLE HELIX |
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| Nucleic Acid Sequence: |
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| Chains | A, B: | |
(DC) (GMS) (DC) (DG) (DC) (DG)
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| Primary Citation: | Wilds, C.J., Pattanayek, R., Pan, C., Wawrzak, Z., Egli, M.
Selenium-Assisted Nucleic Acid Crystallography: Use of Phosphoroselenoates for MAD Phasing of a DNA Structure
J.Am.Chem.Soc.
, 124,
pp. 14910 - 14916, 2002.
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| Experimental Information: | X-RAY DIFFRACTION |
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| Space Group: |
P
21
21
21
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| Cell Constants: |
| a = 17.778 | b = 31.348 | c = 44.116 | (Ångstroms) |
 = 90.00 |  = 90.00 |  = 90.00 | (degrees) |
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| Crystallization Conditions: |
| Method: | | VAPOR DIFFUSION, SITTING DROP |
| Drop: | | DNA, MgCl, sodium cacodylate, spermine tetrahydrochloride |
| Reservoir: | | MPD |
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| Refinement: | The structure was refined using the SHELXL-97 program.
The R value is
9.7
for 9718 reflections
in the resolution range 20.0 to
1.10 Ångstroms
with I > 0.0 sigma(I).
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