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Nucleic Acid Standards

Sugar and Phosphate Constituents

Torsion Angles in Furanose Rings


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                      C2'-endo ribose     C2'-endo deoxyribose    C3'-endo ribose      C3'-endo deoxyribose 

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torsion angle          mean   esd  N       mean   esd  N           mean    esd  N       mean    esd  N 

                       value               value                   value                value

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C4'-O4'-C1'-C2'        339.2 (5.2, 49)     340.7 (6.3, 27)           2.8 (6.1, 24)        6.6 (3.1, 5)   

O4'-C1'-C2'-C3'         35.2 (3.4, 49)      32.8 (4.9, 27)         335.4 (4.9, 24)      333.3 (2.1, 5)   

C1'-C2'-C3'-C4'        324.6 (2.8, 49)     326.9 (3.6, 27)          35.9 (2.8, 24)       35.6 (1.3, 5)   

C2'-C3'-C4'-O4'         24.2 (4.4, 49)      22.6 (4.5, 27)         324.7 (3.0, 24)      327.7 (2.3, 5)   

C3'-C4'-O4'-C1'        357.7 (5.7, 49)     357.7 (6.1, 27)          20.4 (5.1, 24)       16.4 (3.2, 5)   

C5'-C4'-C3'-C2'        263.4 (4.1, 49)     262.0 (4.1, 27)         204.0 (3.1, 24)      207.0 (2.2, 5)   

C5'-C4'-C3'-O3'        147.3 (4.9, 49)     145.2 (4.0, 27)          81.0 (4.4, 24)       84.8 (4.5, 5)   

O4'-C4'-C3'-O3'        268.1 (5.3, 49)     265.8 (4.3, 27)         201.8 (4.2, 24)      205.4 (4.8, 5)   

C4'-O4'-C1'-N1/9       216.6 (5.4, 49)     217.7 (6.7, 27)         241.4 (6.5, 24)      245.6 (2.5, 5)   

O3'-C3'-C2'-O2'        319.7 (4.2, 49)      na                      44.3 (4.5, 24)       na              

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                      C2'-endo ribose/deoxyribose                 C3'-endo ribose/deoxyribose

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                       mean   esd  N                               mean   esd  N 

                       value                                       value   

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O4'-C1'-N1-C2 (anti)   229.8 (18.4, 38)                            195.7 (6.6, 16) 

O4'-C1'-N1-C2 (syn)     63.4 (10.2,  2)                             na

O4'-C1'-N9-C4 (anti)   237.0 (24.3, 18)                            193.3(14.0, 12)

O4'-C1'-N9-C4 (syn)     58.6 (12.0, 15)                             35.6 (0.0,  2)

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                       mean   esd  N    

                       value            

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O5'-C5'-C4'-C3 (-sc)   292.9(12.3, 12)         

O5'-C5'-C4'-C3 (ap)    179.4 (6.4, 22)         

O5'-C5'-C4'-C3 (+sc)    52.5 (5.7, 93)

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