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NDB ID: GDL012    PDB ID: 1D45 


Title:

DNA DODECAMER C-G-C-G-A-A-T-T-C-G-C-G/HOECHST 33258 COMPLEX:-25 DEGREES C, PIPERAZINE DOWN

Molecular Description:

5'-D(*CP*GP*CP*GP*AP*AP*TP*TP*CP*GP*CP*G)-3'
HOECHST 33258
-25 C
PIPERAZINE DOWN

Deposited:

1991-06-04

Released:

1992-04-15

Structural Keywords:

B DOUBLE HELIX

Nucleic Acid Sequence:

Click to show/hide 1 nucleic acid sequences

Protein Sequence:

No Protein Sequence Found

Primary Citation:

Quintana, J.R., Lipanov, A.A., Dickerson, R.E.
Low-temperature crystallographic analyses of the binding of Hoechst 33258 to the double-helical DNA dodecamer C-G-C-G-A-A-T-T-C-G-C-G. 
Biochemistry, 30, pp. 10294 - 10306, 1991.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 21 21 21

Cell Constants:

a = 23.89   b = 38.52   c = 66.34 (Ångstroms)

α = 90.0   β = 90.0   γ = 90.0 (degrees)

Refinement:

The structure was refined using the NUCLSQ program. The R value is 0.152 for 2873 reflections in the resolution range 8.0 to 1.9 Ångstroms with Fobs >  2.0 sigma(Fobs) and with I >  0.0 sigma(I)