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NDB ID: 1F6U    PDB ID: 1F6U 


Title:

NMR STRUCTURE OF THE HIV-1 NUCLEOCAPSID PROTEIN BOUND TO STEM-LOOP SL2 OF THE PSI-RNA PACKAGING SIGNAL. IMPLICATIONS FOR GENOME RECOGNITION

Molecular Description:

NUCLEOCAPSID PROTEIN/RNA COMPLEX

Structural Keywords:

A DOUBLE HELIX, BULGES

Nucleic Acid Sequence:

Click to show/hide 1 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Amarasinghe, G.K., De Guzman, R.N., Turner, R.B., Chancellor, K.J., Wu, Z.R., Summers, M.F.
NMR structure of the HIV-1 nucleocapsid protein bound to stem-loop SL2 of the psi-RNA packaging signal. Implications for genome recognition. 
J.Mol.Biol., 301, pp. 491 - 511, 2000.

Experimental Information:

SOLUTION NMR

Cell Constants:

a = 1.0   b = 1.0   c = 1.0 (Ångstroms)

α = 90.0   β = 90.0   γ = 90.0 (degrees)

Refinement:

The structure was refined using the program. The R value is for reflections in the resolution range to Ångstroms

Number of Models:

Number of Models: 20 Structures

Sample Details:

0.6-1.2 mM complex of Nucleocapsid protein and SL2 RNA; natural abundance, 15N or 13C/15N isotopic labeled 25mM Acetate buffer