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NDB ID: PD0039    PDB ID: 1B96 


Title:

ANALYSIS OF A MUTATIONAL HOT-SPOT IN THE ECORV RESTRICTION ENDONUCLEASE: A CATALYTIC ROLE FOR A MAIN CHAIN CARBONYL GROUP

Molecular Description:

RESTRICTION ENDONUCLEASE ECORV (E.C.3.1.21.4)/DNA COMPLEX

Deposited:

1999-02-19

Released:

1999-02-26

Structural Keywords:

B DOUBLE HELIX, FLIPPED-OUT BASES

Nucleic Acid Sequence:

Click to show/hide 1 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Thomas, M.P., Brady, R.L., Halford, S.E., Sessions, R.B., Baldwin, G.S.
Structural analysis of a mutational hot-spot in the EcoRV restriction endonuclease: a catalytic role for a main chain carbonyl group. 
Nucleic Acids Res., 27, pp. 3438 - 3445, 1999.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 1

Cell Constants:

a = 48.35   b = 48.78   c = 63.64 (Ångstroms)

α = 96.61   β = 108.94   γ = 107.44 (degrees)

Refinement:

The structure was refined using the REFMAC program. The R value is 0.0 for 21703 reflections in the resolution range 12.5 to 2.3 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)