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NDB ID: PD0096    PDB ID: 1CYQ 


Title:

INTRON ENCODED HOMING ENDONUCLEASE I-PPOI (H98A)/DNA HOMING SITE COMPLEX

Molecular Description:

Deposited:

1999-08-31

Released:

1999-11-19

Structural Keywords:

B DOUBLE HELIX, OVERHANGING BASES

Nucleic Acid Sequence:

Click to show/hide 1 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Galburt, E.A., Chevalier, B., Tang, W., Jurica, M.S., Flick, K.E., Monnat Jr., R.J., Stoddard, B.L.
A novel endonuclease mechanism directly visualized for I-PpoI. 
Nat.Struct.Biol., 6, pp. 1096 - 1099, 1999.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 31 2 1

Cell Constants:

a = 113.866   b = 113.866   c = 89.04 (Ångstroms)

α = 90.0   β = 90.0   γ = 120.0 (degrees)

Refinement:

The structure was refined using the X-PLOR program. The R value is 0.204 for 49539 reflections in the resolution range 50.0 to 1.93 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)