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NDB ID: PD0404    PDB ID: 1OUQ 


Title:

CRYSTAL STRUCTURE OF WILD-TYPE CRE RECOMBINASE-LOXP SYNAPSE

Molecular Description:

Cre recombinase/DNA COMPLEX

Deposited:

2003-03-25

Released:

2003-09-16

Structural Keywords:

B DOUBLE HELIX

Nucleic Acid Sequence:

Click to show/hide 3 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Ennifar, E., Meyer, J.E.W., Buchholz, F., Stewart, A.F., Suck, D.
Crystal structure of a wild-type Cre recombinase-loxP synapse reveals a novel spacer conformation suggesting an alternative mechanism for DNA cleavage activation 
Nucleic Acids Res., 31, pp. 5449 - 5460, 2003.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 21 21 21

Cell Constants:

a = 109.5   b = 165.6   c = 193.7 (Ångstroms)

α = 90.0   β = 90.0   γ = 90.0 (degrees)

Refinement:

The structure was refined using the CNS program. The R value is 0.21 for 51908 reflections in the resolution range 8.0 to 3.2 Ångstroms with Fobs >  3.0 sigma(Fobs) and with I >  0.0 sigma(I)