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NDB ID: PD0561    PDB ID: 1U0C 


Title:

Y33C MUTANT OF HOMING ENDONUCLEASE I-CREI

Molecular Description:

DNA endonuclease I-CreI (E.C. 3.1.-.-)/DNA Complex

Deposited:

2004-07-13

Released:

2004-11-02

Structural Keywords:

B DOUBLE HELIX

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Sussman, D., Chadsey, M., Fauce, S., Engel, A., Bruett, A., Monnat, R., Stoddard, B.L., Seligman, L.M.
Isolation and characterization of new homing endonuclease specificities at individual target site positions. 
J.Mol.Biol., 342, pp. 31 - 41, 2004.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 1 21 1

Cell Constants:

a = 43.089   b = 68.021   c = 87.182 (Ångstroms)

α = 90.0   β = 92.0   γ = 90.0 (degrees)

Refinement:

The structure was refined using the CNS program. The R value is 0.22 for 16870 reflections in the resolution range 50.0 to 2.5 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)