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NDB ID: PD0595    PDB ID: 1XHV 


Title:

HINCII BOUND TO CLEAVED COGNATE DNA GTCGAC AND MN2+

Molecular Description:

5'-D(*GP*CP*CP*GP*GP*TP*C)-3'/ 5'-D(P*GP*AP*CP*CP*GP*G)-3'/ Type II restriction enzyme HincII

Deposited:

2004-09-20

Released:

2004-09-28

Structural Keywords:

B DOUBLE HELIX, OVERHANGING BASES, FLIPPED-OUT BASES

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Etzkorn, C., Horton, N.C.
Mechanistic Insights from the Structures of HincII Bound to Cognate DNA Cleaved from Addition of Mg(2+) and Mn(2+) 
J.Mol.Biol., 343, pp. 833 - 849, 2004.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

I 21 21 21

Cell Constants:

a = 66.8   b = 178.2   c = 257.3 (Ångstroms)

α = 90.0   β = 90.0   γ = 90.0 (degrees)

Refinement:

The structure was refined using the CNS program. The R value is 0.0 for 53281 reflections in the resolution range 50.0 to 2.5 Ångstroms with Fobs >  2.0 sigma(Fobs) and with I >  0.0 sigma(I)