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NDB ID: PR0251    PDB ID: 2BH2 


Title:

CRYSTAL STRUCTURE OF E. COLI 5-METHYLURIDINE METHYLTRANSFERASE RUMA IN COMPLEX WITH RIBOSOMAL RNA SUBSTRATE AND S-ADENOSYLHOMOCYSTEINE.

Molecular Description:

23S RRNA (URACIL-5-)-METHYLTRANSFERASE RUMA (E.C.2.1.1.-)

Deposited:

2005-01-06

Released:

2005-03-30

Nucleic Acid Sequence:

Click to show/hide 1 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Lee, T.T., Agarwalla, S., Stroud, R.M.
A Unique RNA Fold in the Ruma-RNA-Cofactor Ternary Complex Contributes to Substrate Selectivity and Enzymatic Function 
Cell(Cambridge,Mass.), 120, pp. 599 - , 2005.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

C 1 2 1

Cell Constants:

a = 190.061   b = 63.542   c = 112.019 (Ångstroms)

α = 90.0   β = 125.15   γ = 90.0 (degrees)

Refinement:

The structure was refined using the REFMAC program. The R value is 0.177 for 56408 reflections in the resolution range 50.0 to 2.15 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)