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NDB ID: 2KEJ    PDB ID: 2KEJ 


Title:

SOLUTION STRUCTURE OF A DIMER OF LAC REPRESSOR DNA-BINDING DOMAIN COMPLEXED TO ITS NATURAL OPERATOR O2

Molecular Description:

Deposited:

2009-01-30

Released:

2009-05-19

Structural Keywords:

B DOUBLE HELIX

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Romanuka, J., Folkers, G.E., Biris, N., Tishchenko, E., Wienk, H., Bonvin, A.M., Kaptein, R., Boelens, R.
Specificity and affinity of Lac repressor for the auxiliary operators O2 and O3 are explained by the structures of their protein-DNA complexes. 
J.Mol.Biol., 390, pp. 478 - 489, 2009.

Experimental Information:

SOLUTION NMR

Space Group:

P 1

Number of Models:

Number of Models: 20 Structures

Sample Details:

0.7 mM DNA (5'-D(*DGP*DAP*DAP*DTP*DTP*DGP*DTP*DGP*DAP*DGP*DCP*DGP*DGP*DAP*DTP*DAP*DAP*DCP*DAP*DAP*DTP*DTP*DT)'), 0.7 mM DNA (5'-D(P*DAP*DAP*DAP*DTP*DTP*DGP*DTP*DTP*DAP*DTP*DCP*DCP*DGP*DCP*DTP*DCP*DAP*DCP*DAP*DAP*DTP*DTP*DC)'), 0.7 mM Lac headpiece dimer, 5% D2O, 10 mM potassium phosphate, 20 mM potassium chloride, 5% U-100% 2H d8-glycerol, 0.01% sodium azide, 95% H2O/5% D2O 95% H2O/5% D2O