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NDB ID: 2MI0    PDB ID: 2MI0 


Title:

NMR STRUCTURE OF THE I-V KISSING-LOOP INTERACTION OF THE NEUROSPORA VS RIBOZYME

Molecular Description:

5'-R(*GP*AP*GP*CP*AP*GP*CP*AP*UP*CP*GP*UP*CP*GP*GP*CP*UP*GP*CP*UP*CP*A)-3'

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

No Protein Sequence Found

Primary Citation:

Bouchard, P., Legault, P.
Structural insights into substrate recognition by the neurospora varkud satellite ribozyme: importance of u-turns at the kissing-loop junction. 
Biochemistry, 53, pp. 258 - 269, 2014.

Experimental Information:

SOLUTION NMR

Cell Constants:

a =   b =   c = (Ångstroms)

α =   β =   γ = (degrees)

Refinement:

The structure was refined using the program. The R value is for reflections in the resolution range to Ångstroms

Number of Models:

Number of Models: 21 Structures

Sample Details:

1.3-2.2 mM 5'-R(*GP*AP*GP*CP*AP*GP*CP*AP*UP*CP*GP*UP*CP*GP*GP*CP*UP*GP*CP*UP*CP*A)-3', 1.3-2.2 mM [U-15N] 5'-R(*GP*CP*GP*GP*CP*AP*GP*UP*UP*GP*AP*CP*UP*AP*CP*UP*GP*UP*CP*GP*C)-3', 10 mM [U-2H] Tris, 50 mM sodium chloride, 0.05 mM sodium azide, 5 mM magnesium chloride, 90% H2O/10% D2O 90% H2O/10% D2O