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NDB ID: 2N8A    PDB ID: 2N8A 


Title:

1H, 13C AND 15N CHEMICAL SHIFT ASSIGNMENTS AND SOLUTION STRUCTURE FOR PARP-1 F1F2 DOMAINS IN COMPLEX WITH A DNA SINGLE-STRAND BREAK

Molecular Description:

Poly [ADP-ribose] polymerase 1 (E.C.2.4.2.30)

Deposited:

2015-10-08

Released:

2015-12-02

Structural Keywords:

B DOUBLE HELIX, BULGE, HAIRPIN LOOP, FLIPPED OUT BASES

Nucleic Acid Sequence:

Click to show/hide 1 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Eustermann, S., Wu, W.F., Langelier, M.F., Yang, J.C., Easton, L.E., Riccio, A.A., Pascal, J.M., Neuhaus, D.
Structural Basis of Detection and Signaling of DNA Single-Strand Breaks by Human PARP-1. 
Mol.Cell, 60, pp. 742 - 754, 2015.

Experimental Information:

SOLUTION NMR

Number of Models:

Number of Models: 78 Structures

Sample Details:

Sample 1: 0.2 mM [U-15N; U-13C; U-2H] PARP-1 1, 0.2 mM DNA (45-MER), 50 mM [U-2H] TRIS, 1 mM [U-2H] DTT, 0.1 mM ZnSO4, 95% H2O/5% D2O 95% H2O/5% D2O