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NDB ID: NA0012    PDB ID: 2VUM 


Title:

ALPHA-AMANITIN INHIBITED COMPLETE RNA POLYMERASE II ELONGATION COMPLEX

Molecular Description:

DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB1 (E.C.2.7.7.6)
DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB2 (E.C.2.7.7.6)
DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB3 (E.C.2.7.7.6)
DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB4 (E.C.2.7.7.6)
DNA-DIRECTED RNA POLYMERASES I
II
AND III SUBUNIT RPABC1 (E.C.2.7.7.6)
DNA-DIRECTED RNA POLYMERASES I
II
AND III SUBUNIT RPABC2 (E.C.2.7.7.6)
DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB7 (E.C.2.7.7.6)
DNA-DIRECTED RNA POLYMERASES I
II
AND III SUBUNIT RPABC3 (E.C.2.7.7.6)
DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB9 (E.C.2.7.7.6)
DNA-DIRECTED RNA POLYMERASES I
II
AND III SUBUNIT RPABC5 (E.C.2.7.7.6)
DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB11 (E.C.2.7.7.6)
DNA-DIRECTED RNA POLYMERASES I
II
AND III SUBUNIT RPABC4
AMATOXIN

Nucleic Acid Sequence:

Click to show/hide 3 nucleic acid sequences

Protein Sequence:

Click to show/hide 13 protein sequences

Primary Citation:

Brueckner, F., Cramer, P.
Structural Basis of Transcription Inhibition by Alpha-Amanitin and Implications for RNA Polymerase II Translocation. 
Nat.Struct.Mol.Biol., 15, pp. 811 - 818, 2008.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

C 2 2 21

Cell Constants:

a = 220.619   b = 394.234   c = 283.966 (Ångstroms)

α = 90.0   β = 90.0   γ = 90.0 (degrees)

Refinement:

The structure was refined using the CNS 1.2 program. The R value is 0.2547 for 328638 reflections in the resolution range 50.0 to 3.4 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)