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NDB ID: PD0988    PDB ID: 2JEJ 


Title:

THE MOLECULAR BASIS OF SELECTIVITY OF NUCLEOSIDE TRIPHOSPHATE INCORPORATION OPPOSITE O6-BENZYLGUANINE BY SULFOLOBUS SOLFATARICUS DNA POLYMERASE IV: STEADY-STATE AND PRE-STEADY-STATE KINETICS AND X- RAY CRYSTALLOGRAPHY OF CORRECT AND INCORRECT PAIRING

Molecular Description:

DNA POLYMERASE IV (E.C.2.7.7.7)

Deposited:

2007-01-17

Released:

2007-03-13

Structural Keywords:

B DOUBLE HELIX, OVERHANGING BASES

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Eoff, R.L., Angel, K.C., Egli, M., Guengerich, F.P.
Molecular Basis of Selectivity of Nucleoside Triphosphate Incorporation Opposite O6-Benzylguanine by Sulfolobus Solfataricus DNA Polymerase Dpo4: Steady-State and Pre-Steady-State Kinetics and X-Ray Crystallography of Correct and Incorrect Pairing. 
J.Biol.Chem., 282, pp. 13573 - , 2007.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 21 21 2

Cell Constants:

a = 95.22   b = 103.855   c = 52.74 (Ångstroms)

α = 90.0   β = 90.0   γ = 90.0 (degrees)

Refinement:

The structure was refined using the CNS program. The R value is 0.238 for 44116 reflections in the resolution range 47.03 to 1.86 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)