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NDB ID: PD1005    PDB ID: 2PI5 


Title:

T7 RNA POLYMERASE COMPLEXED WITH A PHI10 PROMOTER

Molecular Description:

DNA-directed RNA polymerase (E.C. 2.7.7.6)/DNA Complex

Deposited:

2007-04-12

Released:

2007-06-19

Structural Keywords:

B DOUBLE HELIX

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Kennedy, W.P., Momand, J.R., Yin, Y.W.
Mechanism for de novo RNA synthesis and initiating nucleotide specificity by t7 RNA polymerase. 
J.Mol.Biol., 370, pp. 256 - 268, 2007.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 21 21 2

Cell Constants:

a = 224.45   b = 73.791   c = 80.315 (Ångstroms)

α = 90.0   β = 90.0   γ = 90.0 (degrees)

Refinement:

The structure was refined using the CNS program. The R value is 0.249 for 27222 reflections in the resolution range 39.04 to 2.9 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)