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NDB ID: NA0341    PDB ID: 2W9B 


Title:

BINARY COMPLEX OF DPO4 BOUND TO N2,N2-DIMETHYL-DEOXYGUANOSINE MODIFIED DNA

Molecular Description:

DNA POLYMERASE IV (E.C.2.7.7.7)

Deposited:

2009-01-22

Released:

2009-05-12

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

Click to show/hide 2 protein sequences

Primary Citation:

Zhang, H., Eoff, R.L., Kosekov, I.D., Rizzo, C.J., Egli, M., Guengerich, F.P.
Structure-Function Relationships in Miscoding by Sulfolobus Solfataricus DNA Polymerase Dpo4: Guanine N2,N2-Dimethyl Substitution Produces Inactive and Miscoding Polymerase Complexes. 
J.Biol.Chem., 284, pp. 17687 - , 2009.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 1 21 1

Cell Constants:

a = 51.794   b = 101.776   c = 97.268 (Ångstroms)

α = 90.0   β = 91.95   γ = 90.0 (degrees)

Refinement:

The structure was refined using the CNS program. The R value is 0.239 for 44372 reflections in the resolution range 28.75 to 2.28 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)