NDB ID: NA0144    PDB ID: 3JPO     NAKB: NA0144 

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Title:

TERNARY COMPLEX OF DNA POLYMERASE BETA WITH A DIDEOXY TERMINATED PRIMER AND 2'-DEOXYGUANOSINE 5'-BETA, GAMMA-MONOCHLOROMETHYLENE TRIPHOSPHATE

Molecular Description:

DNA polymerase beta (E.C.2.7.7.7)/DNA complex

Deposited:

2009-09-04

Released:

2010-06-02

Structural Keywords:

B DOUBLE HELIX, NICKED

Nucleic Acid Sequence:

Click to show/hide 3 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Batra, V.K., Pedersen, L.C., Beard, W.A., Wilson, S.H., Kashemirov, B.A., Upton, T.G., Goodman, M.F., McKenna, C.E.
Halogenated beta,gamma-Methylene- and Ethylidene-dGTP-DNA Ternary Complexes with DNA Polymerase beta: Structural Evidence for Stereospecific Binding of the Fluoromethylene Analogues. 
J.Am.Chem.Soc., 132, pp. 7617 - 7625, 2010.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 1 21 1

Cell Constants:

a = 50.46   b = 79.99   c = 55.43 (Ångstroms)

α = 90.0   β = 107.91   γ = 90.0 (degrees)

Refinement:

The structure was refined using the CNS program. The R value is 0.194 for 26048 reflections in the resolution range 22.3 to 2.0 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)

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Biological Assembly coordinates (pdb format) 1

Structure Factors (cif format)

Coordinates (pdb format, Unix compressed(.gz))

Coordinates (cif format, Unix compressed(.gz))

NMR Restraints (cif format, Unix compressed(.gz))

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