A Portal for Three-dimensional Structural Information about Nucleic Acids
As of 8-Jul-2020 number of released structures: 10846
Search for released structures

NDB ID: PD1079    PDB ID: 3C2L 


Title:

TERNARY COMPLEX OF DNA POLYMERASE BETA WITH A C:DAPCPP MISMATCH IN THE ACTIVE SITE

Molecular Description:

DNA polymerase beta (E.C.2.7.7.7
4.2.99.-)/DNA Complex

Deposited:

2008-01-25

Released:

2008-05-20

Structural Keywords:

B DOUBLE HELIX, NICKED

Nucleic Acid Sequence:

Click to show/hide 3 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Batra, V.K., Beard, W.A., Shock, D.D., Pedersen, L.C., Wilson, S.H.
Structures of DNA polymerase beta with active-site mismatches suggest a transient abasic site intermediate during misincorporation. 
Mol.Cell, 30, pp. 315 - 324, 2008.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 1 21 1

Cell Constants:

a = 54.47   b = 78.28   c = 54.89 (Ångstroms)

α = 90.0   β = 113.18   γ = 90.0 (degrees)

Refinement:

The structure was refined using the CNS program. The R value is 0.224 for 11355 reflections in the resolution range 23.14 to 2.6 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)