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NDB ID: PD1182    PDB ID: 3ECP 


Title:

CRYSTAL STRUCTURE OF TN5 TRANSPOSASE COMPLEXED WITH 5' PHOSPHORYLATED TRANSPOSON END DNA

Molecular Description:

Deposited:

2008-09-01

Released:

2008-10-21

Structural Keywords:

B DOUBLE HELIX, OVERHANGING BASES

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Klenchin, V.A., Czyz, A., Goryshin, I.Y., Gradman, R., Lovell, S., Rayment, I., Reznikoff, W.S.
Phosphate coordination and movement of DNA in the Tn5 synaptic complex: role of the (R)YREK motif 
Nucleic Acids Res., 36, pp. 5855 - 5862, 2008.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 65 2 2

Cell Constants:

a = 113.508   b = 113.508   c = 228.775 (Ångstroms)

α = 90.0   β = 90.0   γ = 120.0 (degrees)

Refinement:

The structure was refined using the REFMAC program. The R value is 0.22153 for 29291 reflections in the resolution range 30.0 to 2.5 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)