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NDB ID: PD1205    PDB ID: 3FBD 


Title:

CRYSTAL STRUCTURE OF THE NUCLEASE DOMAIN OF COLE7(D493Q MUTANT) IN COMPLEX WITH AN 18-BP DUPLEX DNA

Molecular Description:

Deposited:

2008-11-19

Released:

2009-11-03

Structural Keywords:

B DOUBLE HELIX

Nucleic Acid Sequence:

Click to show/hide 1 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Wang, Y.T., Wright, J.D., Doudeva, L.G., Jhang, H.C., Lim, C., Yuan, H.S.
Redesign of high-affinity nonspecific nucleases with altered sequence preference
To be Published, , pp. - , 0.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 1 21 1

Cell Constants:

a = 60.587   b = 49.423   c = 92.4 (Ångstroms)

α = 90.0   β = 102.64   γ = 90.0 (degrees)

Refinement:

The structure was refined using the CNS program. The R value is 0.201 for 11321 reflections in the resolution range 27.2 to 2.9 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)