## NDB ID: NA0476 PDB ID: 3LZ1

### Title:

CRYSTAL STRUCTURE OF NUCLEOSOME CORE PARTICLE COMPOSED OF THE WIDOM 601 DNA SEQUENCE (ORIENTATION 2)

### Molecular Description:

Histone H3.2

Histone H4

Histone H2A

Histone H2B 1.1/DNA

### Deposited:

2010-03-01

### Released:

2010-09-15

### Structural Keywords:

B DOUBLE HELIX

### Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Chain I

(DA)(DT)(DC)(DG)(DA)(DT)(DG)(DT)(DA)(DT)(DA)(DT)(DA)(DT)(DC)(DT)(DG)(DA)(DC)(DA) (DC)(DG)(

DT)(DG)(DC)(DC)(DT)(DG)(DG)(DA)(DG)(DA)(DC)(DT)(DA)(DG)(DG)(DG)(DA)(DG) (DT)(DA)(DA)(DT)(D

C)(DC)(DC)(DC)(DT)(DT)(DG)(DG)(DC)(DG)(DG)(DT)(DT)(DA)(DA)(DA) (DA)(DC)(DG)(DC)(DG)(DG)(DG

)(DG)(DG)(DA)(DC)(DA)(DG)(DC)(DG)(DC)(DG)(DT)(DA)(DC) (DG)(DT)(DG)(DC)(DG)(DT)(DT)(DT)(DA)

(DA)(DG)(DC)(DG)(DG)(DT)(DG)(DC)(DT)(DA)(DG) (DA)(DG)(DC)(DT)(DG)(DT)(DC)(DT)(DA)(DC)(DG)(

DA)(DC)(DC)(DA)(DA)(DT)(DT)(DG)(DA) (DG)(DC)(DG)(DG)(DC)(DC)(DT)(DC)(DG)(DG)(DC)(DA)(DC)(D

C)(DG)(DG)(DG)(DA)(DT)(DT) (DC)(DT)(DG)(DA)(DT)

Chain J

(DA)(DT)(DC)(DA)(DG)(DA)(DA)(DT)(DC)(DC)(DC)(DG)(DG)(DT)(DG)(DC)(DC)(DG)(DA)(DG) (DG)(DC)(

DC)(DG)(DC)(DT)(DC)(DA)(DA)(DT)(DT)(DG)(DG)(DT)(DC)(DG)(DT)(DA)(DG)(DA) (DC)(DA)(DG)(DC)(D

T)(DC)(DT)(DA)(DG)(DC)(DA)(DC)(DC)(DG)(DC)(DT)(DT)(DA)(DA)(DA) (DC)(DG)(DC)(DA)(DC)(DG)(DT

)(DA)(DC)(DG)(DC)(DG)(DC)(DT)(DG)(DT)(DC)(DC)(DC)(DC) (DC)(DG)(DC)(DG)(DT)(DT)(DT)(DT)(DA)

(DA)(DC)(DC)(DG)(DC)(DC)(DA)(DA)(DG)(DG)(DG) (DG)(DA)(DT)(DT)(DA)(DC)(DT)(DC)(DC)(DC)(DT)(

DA)(DG)(DT)(DC)(DT)(DC)(DC)(DA)(DG) (DG)(DC)(DA)(DC)(DG)(DT)(DG)(DT)(DC)(DA)(DG)(DA)(DT)(D

A)(DT)(DA)(DT)(DA)(DC)(DA) (DT)(DC)(DG)(DA)(DT)

### Protein Sequence:

Click to show/hide 4 protein sequences

Chain A,E

ARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKT DLRFQSSAV

MALQEASEAYLVALFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA

Chain C,G

SGRGKQGGKTRAKAKTRSSRAGLQFPVGRVHRLLRKGNYAERVGAGAPVYLAAVLEYLTAEILELAGNAARDNKKTRIIP RHLQLAVRN

DEELNKLLGRVTIAQGGVLPNIQSVLLPKK

Chain B,F

SGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKVFLENVIRDAVTYTEHAKRKT VTAMDVVYA

LKRQGRTLYGFGG

Chain D,H

PEPAKSAPAPKKGSKKAVTKTQKKDGKKRRKTRKESYAIYVYKVLKQVHPDTGISSKAMSIMNSFVNDVFERIAGEASRL AHYNKRSTI

TSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSAK

### Primary Citation:

Vasudevan, D., Chua, E.Y., Davey, C.A.

Crystal structures of nucleosome core particles containing the '601' strong positioning sequence

*J.Mol.Biol.*, **403**, pp. 1 - 10, 2010.

### Experimental Information:

X-RAY DIFFRACTION

### Space Group:

P 21 21 21

### Cell Constants:

a = 107.37 b = 109.66 c = 175.75 (Ångstroms)

α = 90.0 β = 90.0 γ = 90.0 (degrees)

### Refinement:

The structure was refined using the REFMAC program.
The R value is 0.27 for 65180 reflections in
the resolution range 93.04 to 2.5 Ångstroms
with Fobs > 0.0 sigma(Fobs) and with I > 0.0 sigma(I)