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NDB ID: NA0583    PDB ID: 3NAE 


Title:

RB69 DNA POLYMERASE (Y567A) TERNARY COMPLEX WITH DATP OPPOSITE GUANIDINOHYDANTOIN

Molecular Description:

DNA polymerase (E.C.2.7.7.7)/DNA complex

Deposited:

2010-06-01

Released:

2010-09-15

Structural Keywords:

B DOUBLE HELIX, OVERHANGING BASES

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Beckman, J., Wang, M., Blaha, G., Wang, J., Konigsberg, W.H.
Substitution of Ala for Tyr567 in RB69 DNA Polymerase Allows dAMP and dGMP To Be Inserted opposite Guanidinohydantoin . 
Biochemistry, 49, pp. 8554 - 8563, 2010.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 21 21 21

Cell Constants:

a = 75.013   b = 119.783   c = 130.727 (Ångstroms)

α = 90.0   β = 90.0   γ = 90.0 (degrees)

Refinement:

The structure was refined using the REFMAC program. The R value is 0.19596 for 69794 reflections in the resolution range 35.24 to 2.003 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  1.0 sigma(I)