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NDB ID: 4PAR    PDB ID: 4PAR 


Title:

THE 5-HYDROXYMETHYLCYTOSINE-SPECIFIC RESTRICTION ENZYME ABASI IN A COMPLEX WITH PRODUCT-LIKE DNA

Molecular Description:

AbaSI/DNA Complex

Deposited:

2014-04-09

Released:

2014-06-25

Structural Keywords:

B DOUBLE HELIX

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Horton, J.R., Borgaro, J.G., Griggs, R.M., Quimby, A., Guan, S., Zhang, X., Wilson, G.G., Zheng, Y., Zhu, Z., Cheng, X.
Structure of 5-hydroxymethylcytosine-specific restriction enzyme, AbaSI, in complex with DNA. 
Nucleic Acids Res., 42, pp. 7947 - 7959, 2014.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 1 21 1

Cell Constants:

a = 54.826   b = 144.704   c = 105.033 (Ångstroms)

α = 90.0   β = 94.57   γ = 90.0 (degrees)

Refinement:

The structure was refined using the PHENIX program. The R value is 0.1927 for 35850 reflections in the resolution range 34.9 to 2.89 Ångstroms with Fobs >  1.35 sigma(Fobs) and with I >  0.0 sigma(I)