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NDB ID: 4PKD    PDB ID: 4PKD 


Title:

U1-70K IN COMPLEX WITH U1 SNRNA STEM-LOOPS 1 AND U1-A RRM IN COMPLEX WITH STEM-LOOP 2

Molecular Description:

U1-A (2-111) plus (Gly-Ser)6-Gly linker
U1-70k (60-215)/RNA Complex

Deposited:

2014-05-14

Released:

2014-12-31

Structural Keywords:

A DOUBLE HELIX, HAIRPIN LOOP

Nucleic Acid Sequence:

Click to show/hide 1 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Kondo, Y., Oubridge, C., van Roon, A.M., Nagai, K.
Crystal structure of human U1 snRNP, a small nuclear ribonucleoprotein particle, reveals the mechanism of 5' splice site recognition. 
Elife, 4, pp. - , 2015.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

C 1 2 1

Cell Constants:

a = 80.22   b = 66.6   c = 93.73 (Ångstroms)

α = 90.0   β = 110.95   γ = 90.0 (degrees)

Refinement:

The structure was refined using the REFMAC program. The R value is 0.19992 for 15156 reflections in the resolution range 87.0 to 2.5 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)